cell lines hbec 3kt gift Search Results


hbec 3kt  (ATCC)
99
ATCC hbec 3kt
Hbec 3kt, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nb100-91995  (Bio-Techne corporation)
91
Bio-Techne corporation nb100-91995
Nb100 91995, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human bronchial epithelial hbe 3 kt cells  (ATCC)
97
ATCC human bronchial epithelial hbe 3 kt cells
IFN-λ inhibited SARS-CoV-2 replication in cultured human lung cells. Human bronchial <t>epithelial</t> cells expressing hACE2 <t>(HBE-hACE2)</t> were either mock treated (black-filled circles), pretreated for 24 h with IFN-λ (pre-IFN-λ) (green open squares), or treated with IFN-λ at the same time of infection (co-IFN-λ) (green-filled circles) with SARS-CoV-2. Culture medium was harvested at 0, 24, and 48 h postinfection, and viral RNA in culture medium was measured by RT-qPCR following infection at MOI of 0.01 PFU/cell (A) or MOI of 0.1 PFU/cell (B). Mature virus released into the culture media was quantified by plaque assay following infection at MOI of 0.01 PFU/cell (C) or MOI of 0.1 PFU/cell (D). Three independent experiments were performed with technical duplicates. * , P < 0.001 (two-way ANOVA). (E) Representative Western blots are shown of proteins expressed in the HBE and HBE-hACE2 cells following treatment with increasing amounts of IFN-λ (200 to 2,000 units/ml) for 24 h. (F) RT-qPCR measurements are shown for ISG mRNAs expressed in HBE-hACE2 cells relative to untreated controls in response to IFN-λ treatment (1,000 units/ml) or relative to mock treatment in response to a SARS-CoV-2 infection for 24 h.
Human Bronchial Epithelial Hbe 3 Kt Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 97 stars, based on 1 article reviews
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human bronchial epithelial cell line hbec-3kt  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare human bronchial epithelial cell line hbec-3kt
IFN-λ inhibited SARS-CoV-2 replication in cultured human lung cells. Human bronchial <t>epithelial</t> cells expressing hACE2 <t>(HBE-hACE2)</t> were either mock treated (black-filled circles), pretreated for 24 h with IFN-λ (pre-IFN-λ) (green open squares), or treated with IFN-λ at the same time of infection (co-IFN-λ) (green-filled circles) with SARS-CoV-2. Culture medium was harvested at 0, 24, and 48 h postinfection, and viral RNA in culture medium was measured by RT-qPCR following infection at MOI of 0.01 PFU/cell (A) or MOI of 0.1 PFU/cell (B). Mature virus released into the culture media was quantified by plaque assay following infection at MOI of 0.01 PFU/cell (C) or MOI of 0.1 PFU/cell (D). Three independent experiments were performed with technical duplicates. * , P < 0.001 (two-way ANOVA). (E) Representative Western blots are shown of proteins expressed in the HBE and HBE-hACE2 cells following treatment with increasing amounts of IFN-λ (200 to 2,000 units/ml) for 24 h. (F) RT-qPCR measurements are shown for ISG mRNAs expressed in HBE-hACE2 cells relative to untreated controls in response to IFN-λ treatment (1,000 units/ml) or relative to mock treatment in response to a SARS-CoV-2 infection for 24 h.
Human Bronchial Epithelial Cell Line Hbec 3kt, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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hbec-3kt cell line  (Purdue University Cytometry)
90
Purdue University Cytometry hbec-3kt cell line
IFN-λ inhibited SARS-CoV-2 replication in cultured human lung cells. Human bronchial <t>epithelial</t> cells expressing hACE2 <t>(HBE-hACE2)</t> were either mock treated (black-filled circles), pretreated for 24 h with IFN-λ (pre-IFN-λ) (green open squares), or treated with IFN-λ at the same time of infection (co-IFN-λ) (green-filled circles) with SARS-CoV-2. Culture medium was harvested at 0, 24, and 48 h postinfection, and viral RNA in culture medium was measured by RT-qPCR following infection at MOI of 0.01 PFU/cell (A) or MOI of 0.1 PFU/cell (B). Mature virus released into the culture media was quantified by plaque assay following infection at MOI of 0.01 PFU/cell (C) or MOI of 0.1 PFU/cell (D). Three independent experiments were performed with technical duplicates. * , P < 0.001 (two-way ANOVA). (E) Representative Western blots are shown of proteins expressed in the HBE and HBE-hACE2 cells following treatment with increasing amounts of IFN-λ (200 to 2,000 units/ml) for 24 h. (F) RT-qPCR measurements are shown for ISG mRNAs expressed in HBE-hACE2 cells relative to untreated controls in response to IFN-λ treatment (1,000 units/ml) or relative to mock treatment in response to a SARS-CoV-2 infection for 24 h.
Hbec 3kt Cell Line, supplied by Purdue University Cytometry, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hbec 3kt-rl cells  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology hbec 3kt-rl cells
IFN-λ inhibited SARS-CoV-2 replication in cultured human lung cells. Human bronchial <t>epithelial</t> cells expressing hACE2 <t>(HBE-hACE2)</t> were either mock treated (black-filled circles), pretreated for 24 h with IFN-λ (pre-IFN-λ) (green open squares), or treated with IFN-λ at the same time of infection (co-IFN-λ) (green-filled circles) with SARS-CoV-2. Culture medium was harvested at 0, 24, and 48 h postinfection, and viral RNA in culture medium was measured by RT-qPCR following infection at MOI of 0.01 PFU/cell (A) or MOI of 0.1 PFU/cell (B). Mature virus released into the culture media was quantified by plaque assay following infection at MOI of 0.01 PFU/cell (C) or MOI of 0.1 PFU/cell (D). Three independent experiments were performed with technical duplicates. * , P < 0.001 (two-way ANOVA). (E) Representative Western blots are shown of proteins expressed in the HBE and HBE-hACE2 cells following treatment with increasing amounts of IFN-λ (200 to 2,000 units/ml) for 24 h. (F) RT-qPCR measurements are shown for ISG mRNAs expressed in HBE-hACE2 cells relative to untreated controls in response to IFN-λ treatment (1,000 units/ml) or relative to mock treatment in response to a SARS-CoV-2 infection for 24 h.
Hbec 3kt Rl Cells, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cms-3kt  (Xingcheng Chempharm Co Ltd)
90
Xingcheng Chempharm Co Ltd cms-3kt
IFN-λ inhibited SARS-CoV-2 replication in cultured human lung cells. Human bronchial <t>epithelial</t> cells expressing hACE2 <t>(HBE-hACE2)</t> were either mock treated (black-filled circles), pretreated for 24 h with IFN-λ (pre-IFN-λ) (green open squares), or treated with IFN-λ at the same time of infection (co-IFN-λ) (green-filled circles) with SARS-CoV-2. Culture medium was harvested at 0, 24, and 48 h postinfection, and viral RNA in culture medium was measured by RT-qPCR following infection at MOI of 0.01 PFU/cell (A) or MOI of 0.1 PFU/cell (B). Mature virus released into the culture media was quantified by plaque assay following infection at MOI of 0.01 PFU/cell (C) or MOI of 0.1 PFU/cell (D). Three independent experiments were performed with technical duplicates. * , P < 0.001 (two-way ANOVA). (E) Representative Western blots are shown of proteins expressed in the HBE and HBE-hACE2 cells following treatment with increasing amounts of IFN-λ (200 to 2,000 units/ml) for 24 h. (F) RT-qPCR measurements are shown for ISG mRNAs expressed in HBE-hACE2 cells relative to untreated controls in response to IFN-λ treatment (1,000 units/ml) or relative to mock treatment in response to a SARS-CoV-2 infection for 24 h.
Cms 3kt, supplied by Xingcheng Chempharm Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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v-3kt 2000 l/s turbo molecular pump  (Varian Medical)
90
Varian Medical v-3kt 2000 l/s turbo molecular pump
IFN-λ inhibited SARS-CoV-2 replication in cultured human lung cells. Human bronchial <t>epithelial</t> cells expressing hACE2 <t>(HBE-hACE2)</t> were either mock treated (black-filled circles), pretreated for 24 h with IFN-λ (pre-IFN-λ) (green open squares), or treated with IFN-λ at the same time of infection (co-IFN-λ) (green-filled circles) with SARS-CoV-2. Culture medium was harvested at 0, 24, and 48 h postinfection, and viral RNA in culture medium was measured by RT-qPCR following infection at MOI of 0.01 PFU/cell (A) or MOI of 0.1 PFU/cell (B). Mature virus released into the culture media was quantified by plaque assay following infection at MOI of 0.01 PFU/cell (C) or MOI of 0.1 PFU/cell (D). Three independent experiments were performed with technical duplicates. * , P < 0.001 (two-way ANOVA). (E) Representative Western blots are shown of proteins expressed in the HBE and HBE-hACE2 cells following treatment with increasing amounts of IFN-λ (200 to 2,000 units/ml) for 24 h. (F) RT-qPCR measurements are shown for ISG mRNAs expressed in HBE-hACE2 cells relative to untreated controls in response to IFN-λ treatment (1,000 units/ml) or relative to mock treatment in response to a SARS-CoV-2 infection for 24 h.
V 3kt 2000 L/S Turbo Molecular Pump, supplied by Varian Medical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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carbon molecular sieves cms takeda 3kt  (Takeda)
90
Takeda carbon molecular sieves cms takeda 3kt
IFN-λ inhibited SARS-CoV-2 replication in cultured human lung cells. Human bronchial <t>epithelial</t> cells expressing hACE2 <t>(HBE-hACE2)</t> were either mock treated (black-filled circles), pretreated for 24 h with IFN-λ (pre-IFN-λ) (green open squares), or treated with IFN-λ at the same time of infection (co-IFN-λ) (green-filled circles) with SARS-CoV-2. Culture medium was harvested at 0, 24, and 48 h postinfection, and viral RNA in culture medium was measured by RT-qPCR following infection at MOI of 0.01 PFU/cell (A) or MOI of 0.1 PFU/cell (B). Mature virus released into the culture media was quantified by plaque assay following infection at MOI of 0.01 PFU/cell (C) or MOI of 0.1 PFU/cell (D). Three independent experiments were performed with technical duplicates. * , P < 0.001 (two-way ANOVA). (E) Representative Western blots are shown of proteins expressed in the HBE and HBE-hACE2 cells following treatment with increasing amounts of IFN-λ (200 to 2,000 units/ml) for 24 h. (F) RT-qPCR measurements are shown for ISG mRNAs expressed in HBE-hACE2 cells relative to untreated controls in response to IFN-λ treatment (1,000 units/ml) or relative to mock treatment in response to a SARS-CoV-2 infection for 24 h.
Carbon Molecular Sieves Cms Takeda 3kt, supplied by Takeda, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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engineered, acid-tolerant yeast strain  (Cargill Inc)
90
Cargill Inc engineered, acid-tolerant yeast strain
IFN-λ inhibited SARS-CoV-2 replication in cultured human lung cells. Human bronchial <t>epithelial</t> cells expressing hACE2 <t>(HBE-hACE2)</t> were either mock treated (black-filled circles), pretreated for 24 h with IFN-λ (pre-IFN-λ) (green open squares), or treated with IFN-λ at the same time of infection (co-IFN-λ) (green-filled circles) with SARS-CoV-2. Culture medium was harvested at 0, 24, and 48 h postinfection, and viral RNA in culture medium was measured by RT-qPCR following infection at MOI of 0.01 PFU/cell (A) or MOI of 0.1 PFU/cell (B). Mature virus released into the culture media was quantified by plaque assay following infection at MOI of 0.01 PFU/cell (C) or MOI of 0.1 PFU/cell (D). Three independent experiments were performed with technical duplicates. * , P < 0.001 (two-way ANOVA). (E) Representative Western blots are shown of proteins expressed in the HBE and HBE-hACE2 cells following treatment with increasing amounts of IFN-λ (200 to 2,000 units/ml) for 24 h. (F) RT-qPCR measurements are shown for ISG mRNAs expressed in HBE-hACE2 cells relative to untreated controls in response to IFN-λ treatment (1,000 units/ml) or relative to mock treatment in response to a SARS-CoV-2 infection for 24 h.
Engineered, Acid Tolerant Yeast Strain, supplied by Cargill Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IFN-λ inhibited SARS-CoV-2 replication in cultured human lung cells. Human bronchial epithelial cells expressing hACE2 (HBE-hACE2) were either mock treated (black-filled circles), pretreated for 24 h with IFN-λ (pre-IFN-λ) (green open squares), or treated with IFN-λ at the same time of infection (co-IFN-λ) (green-filled circles) with SARS-CoV-2. Culture medium was harvested at 0, 24, and 48 h postinfection, and viral RNA in culture medium was measured by RT-qPCR following infection at MOI of 0.01 PFU/cell (A) or MOI of 0.1 PFU/cell (B). Mature virus released into the culture media was quantified by plaque assay following infection at MOI of 0.01 PFU/cell (C) or MOI of 0.1 PFU/cell (D). Three independent experiments were performed with technical duplicates. * , P < 0.001 (two-way ANOVA). (E) Representative Western blots are shown of proteins expressed in the HBE and HBE-hACE2 cells following treatment with increasing amounts of IFN-λ (200 to 2,000 units/ml) for 24 h. (F) RT-qPCR measurements are shown for ISG mRNAs expressed in HBE-hACE2 cells relative to untreated controls in response to IFN-λ treatment (1,000 units/ml) or relative to mock treatment in response to a SARS-CoV-2 infection for 24 h.

Journal: mBio

Article Title: Interferon-Lambda Intranasal Protection and Differential Sex Pathology in a Murine Model of SARS-CoV-2 Infection

doi: 10.1128/mBio.02756-21

Figure Lengend Snippet: IFN-λ inhibited SARS-CoV-2 replication in cultured human lung cells. Human bronchial epithelial cells expressing hACE2 (HBE-hACE2) were either mock treated (black-filled circles), pretreated for 24 h with IFN-λ (pre-IFN-λ) (green open squares), or treated with IFN-λ at the same time of infection (co-IFN-λ) (green-filled circles) with SARS-CoV-2. Culture medium was harvested at 0, 24, and 48 h postinfection, and viral RNA in culture medium was measured by RT-qPCR following infection at MOI of 0.01 PFU/cell (A) or MOI of 0.1 PFU/cell (B). Mature virus released into the culture media was quantified by plaque assay following infection at MOI of 0.01 PFU/cell (C) or MOI of 0.1 PFU/cell (D). Three independent experiments were performed with technical duplicates. * , P < 0.001 (two-way ANOVA). (E) Representative Western blots are shown of proteins expressed in the HBE and HBE-hACE2 cells following treatment with increasing amounts of IFN-λ (200 to 2,000 units/ml) for 24 h. (F) RT-qPCR measurements are shown for ISG mRNAs expressed in HBE-hACE2 cells relative to untreated controls in response to IFN-λ treatment (1,000 units/ml) or relative to mock treatment in response to a SARS-CoV-2 infection for 24 h.

Article Snippet: Human bronchial epithelial (HBE) 3-KT cells were obtained from American Type Culture Collection (ATCC) and cultured in airway epithelial cell basal medium supplemented with the bronchial epithelial cell growth kit (ATCC).

Techniques: Cell Culture, Expressing, Infection, Quantitative RT-PCR, Virus, Plaque Assay, Western Blot